Detailed information of TA system TA180903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 815147..815743
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IS178_RS03705
• Protein ID: WP_016783373.1
• Coordinates: 815441..815743 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H2IG99
• Locus tag: IS178_RS03700
• Protein ID: WP_016783372.1
• Coordinates: 815147..815437 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS03680	811520..813577	-	2058	WP_016783368.1	TonB-dependent copper receptor	-
IS178_RS03685	813676..814047	-	372	WP_016783369.1	DUF2946 domain-containing protein	-
IS178_RS03690	814096..814590	-	495	WP_016783370.1	copper chaperone PCu(A)C	-
IS178_RS03695	814639..815037	-	399	WP_016783371.1	DUF2946 domain-containing protein	-
IS178_RS03700	815147..815437	-	291	WP_016783372.1	putative addiction module antidote protein	Antitoxin
IS178_RS03705	815441..815743	-	303	WP_016783373.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS178_RS03710	815793..816521	-	729	WP_016783374.1	cobalt-precorrin-6A reductase	-
IS178_RS03715	816518..817615	-	1098	WP_016783375.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
IS178_RS03720	817608..818819	-	1212	WP_029611713.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ureB	756550..965926	209376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (2-95)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.99 Da
• Isoelectric Point: 9.4668

>T180903 WP_016783373.1 NZ_CP064354:c815743-815441 [Pseudomonas fragi]
MITFEQTREFAKWLSALKDKTGQARILARLKAAGLGHFADCECVGGGVYEMRIHCGPGYRVYYTRKGEVFYLLLVGGDKT
TQTRDIKRAKQMAHTLGNED

Nucleotide

• Length: 303 bp

>T180903 NZ_CP085785:c1234332-1234078 [Synechococcus elongatus PCC 6301]
ATGAAAGTTGTTTCCTTCAGTGACGCCAGAAAAAATCTCAAGACTGTCTTGGATGAAGTCGTCAACGACGCTGACTACAC
GATCATTACTCGCCGCAATGCCGAGGAAGTCGTGGTCATGTCCCTCGACTCCTTCAATAGCCTGATCGAAACCTTCCACC
TGCTCAAATCCCCTGCCAATGCTGCTCACCTACAACGCTCGATCGCTCAGTACCAGCAAGGTCAAACAGTCGAGCGAAAT
CTATTAGATGCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10349.81 Da
• Isoelectric Point: 4.7824

>AT180903 WP_016783372.1 NZ_CP064354:c815437-815147 [Pseudomonas fragi]
MTEQLTRFDTVDYLKSPEDMAAYLDACFEEDPGDGSLIRAALNDIARAQGMTQIARDAGLGRESLYKALGSKGNPEFATI
IKVMKALGLKLHATAA

Nucleotide

• Length: 291 bp

>AT180903 NZ_CP085785:c1234085-1233822 [Synechococcus elongatus PCC 6301]
ATGCGTAAGCTGGCTTGGACAAACGAGGCTTGGGAAGATTACCTGTATTGGCAAGGGCAGGACAAGAAGACCTTAAATCG
CATCAACAAGCTCATTACCGAAACCTTGCGATCGCCCTTTGAGGGGATTGGTAAGCCAGAAGCGCTCAGGGAGAACCTGA
CTGGGTTTTGGTCACGCCGCATTGACGACACCAATCGCTTAGTTTACGCAGTAGCAGATGACTACCTGACCATTATTTCC
TGTCGCTACCACTACAGCGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1H2IG99