Detailed information of TA system TA180902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 456093..456697
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IS178_RS02145
• Protein ID: WP_081481088.1
• Coordinates: 456093..456290 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A494R758
• Locus tag: IS178_RS02150
• Protein ID: WP_016783077.1
• Coordinates: 456287..456697 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS02130	451851..454289	-	2439	WP_016783073.1	TonB-dependent siderophore receptor	-
IS178_RS02135	454402..455337	-	936	WP_029611678.1	FecR domain-containing protein	-
IS178_RS02140	455349..455849	-	501	WP_016783075.1	sigma-70 family RNA polymerase sigma factor	-
IS178_RS02145	456093..456290	+	198	WP_081481088.1	type II toxin-antitoxin system HicA family toxin	Toxin
IS178_RS02150	456287..456697	+	411	WP_016783077.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IS178_RS02160	456934..457842	-	909	WP_029611679.1	LysR family transcriptional regulator	-
IS178_RS02165	457936..459018	+	1083	WP_016783079.1	tartrate dehydrogenase	-
IS178_RS02170	459316..460953	+	1638	WP_016783080.1	BCCT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7226.44 Da
• Isoelectric Point: 11.0468

>T180902 WP_081481088.1 NZ_CP064354:456093-456290 [Pseudomonas fragi]
MRSRELIELLMADGWFEIAVKGSHHQFKHPAKAGRVTVPHPRTEIAKGTLHSILKSAGLKGELRS

Nucleotide

• Length: 198 bp

>T180902 NZ_CP085785:c1234085-1233822 [Synechococcus elongatus PCC 6301]
ATGCGTAAGCTGGCTTGGACAAACGAGGCTTGGGAAGATTACCTGTATTGGCAAGGGCAGGACAAGAAGACCTTAAATCG
CATCAACAAGCTCATTACCGAAACCTTGCGATCGCCCTTTGAGGGGATTGGTAAGCCAGAAGCGCTCAGGGAGAACCTGA
CTGGGTTTTGGTCACGCCGCATTGACGACACCAATCGCTTAGTTTACGCAGTAGCAGATGACTACCTGACCATTATTTCC
TGTCGCTACCACTACAGCGATTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14844.63 Da
• Isoelectric Point: 4.4720

>AT180902 WP_016783077.1 NZ_CP064354:456287-456697 [Pseudomonas fragi]
MKFPVVIHKDADSEFSVTVPDVPGCFSAGATFSQALDNVQEALALHLEGIVADGGELPQGKDVDAHLENPDYVGGIWAIV
DFDLTPYLGKSVRFNASLPENLLQRIDERVSRDHRYASRSGFLATAALRELSVQNF

Nucleotide

• Length: 411 bp

>AT180902 NZ_CP085785:c1234332-1234078 [Synechococcus elongatus PCC 6301]
ATGAAAGTTGTTTCCTTCAGTGACGCCAGAAAAAATCTCAAGACTGTCTTGGATGAAGTCGTCAACGACGCTGACTACAC
GATCATTACTCGCCGCAATGCCGAGGAAGTCGTGGTCATGTCCCTCGACTCCTTCAATAGCCTGATCGAAACCTTCCACC
TGCTCAAATCCCCTGCCAATGCTGCTCACCTACAACGCTCGATCGCTCAGTACCAGCAAGGTCAAACAGTCGAGCGAAAT
CTATTAGATGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A494R758