Detailed information of TA system TA180898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4447898..4448484
• Replicon: chromosome
• Accession: NZ_CP064352
• Organism: Klebsiella pneumoniae subsp. pneumoniae ATCC 43816

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: IT767_RS21200
• Protein ID: WP_002920800.1
• Coordinates: 4448116..4448484 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: IT767_RS21195
• Protein ID: WP_004174006.1
• Coordinates: 4447898..4448119 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT767_RS21175	4444055..4444981	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
IT767_RS21180	4444978..4446255	+	1278	WP_038432515.1	branched chain amino acid ABC transporter permease LivM	-
IT767_RS21185	4446252..4447019	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
IT767_RS21190	4447021..4447734	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
IT767_RS21195	4447898..4448119	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IT767_RS21200	4448116..4448484	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IT767_RS21205	4448757..4449275	+	519	Protein_4141	extracellular solute-binding protein	-
IT767_RS21210	4450261..4451148	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
IT767_RS21215	4451145..4451990	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
IT767_RS21220	4451992..4453062	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4442884..4453799	10915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T180898 WP_002920800.1 NZ_CP064352:4448116-4448484 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T180898 NZ_CP085780:3521695-3521994 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT180898 WP_004174006.1 NZ_CP064352:4447898-4448119 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT180898 NZ_CP085780:3522005-3522370 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7