Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4046847..4047472 Replicon chromosome
Accession NZ_CP064352
Organism Klebsiella pneumoniae subsp. pneumoniae ATCC 43816

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IT767_RS19345 Protein ID WP_002882817.1
Coordinates 4046847..4047230 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IT767_RS19350 Protein ID WP_004150355.1
Coordinates 4047230..4047472 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IT767_RS19330 4044213..4045115 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IT767_RS19335 4045112..4045747 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IT767_RS19340 4045744..4046673 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IT767_RS19345 4046847..4047230 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IT767_RS19350 4047230..4047472 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IT767_RS19355 4047677..4048594 + 918 WP_004181612.1 alpha/beta hydrolase -
IT767_RS19360 4048608..4049549 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IT767_RS19365 4049594..4050030 - 437 Protein_3779 D-tyrosyl-tRNA(Tyr) deacylase -
IT767_RS19370 4050027..4050887 - 861 WP_004146232.1 virulence factor BrkB family protein -
IT767_RS19375 4050881..4051480 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180897 WP_002882817.1 NZ_CP064352:c4047230-4046847 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180897 NZ_CP085780:c2728964-2728861 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180897 WP_004150355.1 NZ_CP064352:c4047472-4047230 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180897 NZ_CP085780:2728848-2729057 [Enterobacter hormaechei]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTA
AAAGTGGTCTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References