Detailed information of TA system TA180883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 592704..593347
• Replicon: chromosome
• Accession: NZ_CP064352
• Organism: Klebsiella pneumoniae subsp. pneumoniae ATCC 43816

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: IT767_RS02660
• Protein ID: WP_015874977.1
• Coordinates: 592704..593120 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: IT767_RS02665
• Protein ID: WP_015874976.1
• Coordinates: 593117..593347 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT767_RS02645	588107..589222	-	1116	WP_032447702.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
IT767_RS02650	590094..592268	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
IT767_RS02655	592423..592668	-	246	WP_032447699.1	hypothetical protein	-
IT767_RS02660	592704..593120	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
IT767_RS02665	593117..593347	-	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IT767_RS02670	593790..594170	+	381	WP_015874975.1	YrzE family protein	-
IT767_RS02675	594307..595260	+	954	Protein_522	IS21 family transposase	-
IT767_RS02680	595355..596081	+	727	Protein_523	IS21-like element IS100kyp family helper ATPase IstB	-
IT767_RS02685	596125..596238	-	114	WP_015874970.1	hypothetical protein	-
IT767_RS02690	596481..596690	-	210	WP_038433206.1	TraR/DksA family transcriptional regulator	-
IT767_RS02695	596680..597261	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
IT767_RS02700	597246..597428	-	183	WP_071882703.1	hypothetical protein	-
IT767_RS02705	597450..597635	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	rmpA / iroD / iroC / iroB / iroN	554439..598701	44262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T180883 WP_015874977.1 NZ_CP064352:c593120-592704 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T180883 NZ_CP085771:c100465-99980 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCATCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT180883 WP_015874976.1 NZ_CP064352:c593347-593117 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180883 NZ_CP085771:c100719-100453 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4