Detailed information of TA system TA180864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 552585..554410
• Replicon: chromosome
• Accession: NZ_CP064350
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: IT766_RS02605
• Protein ID: WP_000392003.1
• Coordinates: 553049..554410 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IT766_RS02600
• Protein ID: WP_000804849.1
• Coordinates: 552585..553052 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT766_RS02585	547865..548569	-	705	WP_001880605.1	NERD domain-containing protein	-
IT766_RS02590	548639..551575	-	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
IT766_RS02595	551579..552157	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
IT766_RS02600	552585..553052	-	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
IT766_RS02605	553049..554410	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
IT766_RS02610	555014..555991	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
IT766_RS02615	556017..556697	-	681	Protein_465	IS5 family transposase	-
IT766_RS02620	557039..558559	-	1521	WP_000640055.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T180864 WP_000392003.1 NZ_CP064350:c554410-553049 [Vibrio cholerae C6706]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T180864 NZ_CP085763:81487-81897 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT180864 WP_000804849.1 NZ_CP064350:c553052-552585 [Vibrio cholerae C6706]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT180864 NZ_CP085763:81260-81490 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8