Detailed information of TA system TA180863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 25546..26065
• Replicon: plasmid unnamed3
• Accession: NZ_CP064346
• Organism: Enterococcus faecium strain VRE3363

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: IU206_RS15190
• Protein ID: WP_000588503.1
• Coordinates: 25808..26065 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: IU206_RS15185
• Protein ID: WP_000388479.1
• Coordinates: 25546..25815 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IU206_RS15150	21192..21608	-	417	WP_001232859.1	hypothetical protein	-
IU206_RS15155	21611..21895	-	285	WP_000353844.1	hypothetical protein	-
IU206_RS15160	22240..22485	+	246	WP_002311817.1	hypothetical protein	-
IU206_RS15165	22787..22882	-	96	WP_106913803.1	type I toxin-antitoxin system Fst family toxin	-
IU206_RS15170	23533..24213	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
IU206_RS15175	24289..24855	+	567	Protein_26	hypothetical protein	-
IU206_RS15180	25236..25415	+	180	WP_001120991.1	hypothetical protein	-
IU206_RS15185	25546..25815	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IU206_RS15190	25808..26065	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
IU206_RS15195	26703..27290	+	588	Protein_30	hypothetical protein	-
IU206_RS15200	27445..28764	+	1320	WP_000202380.1	IS1380-like element IS1678 family transposase	-
IU206_RS15205	28968..29210	+	243	Protein_32	hypothetical protein	-
IU206_RS15210	29375..29989	-	615	WP_001261742.1	recombinase family protein	-
IU206_RS15215	30227..30649	+	423	WP_002350734.1	hypothetical protein	-
IU206_RS15220	30659..30862	+	204	WP_002299573.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX	-	1..40064	40064
	-	inside	IScluster/Tn	VanHAX	-	1657..28764	27107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T180863 WP_000588503.1 NZ_CP064346:25808-26065 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T180863 NZ_CP085763:c44151-43666 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT180863 WP_000388479.1 NZ_CP064346:25546-25815 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT180863 NZ_CP085763:c44405-44139 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7