Detailed information of TA system TA180862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 4125..5262
• Replicon: plasmid unnamed1
• Accession: NZ_CP064344
• Organism: Enterococcus faecium strain VRE3363

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: IU206_RS13495
• Protein ID: WP_002401483.1
• Coordinates: 4125..4988 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: IU206_RS13500
• Protein ID: WP_000301765.1
• Coordinates: 4990..5262 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IU206_RS13465	6..1496	-	1491	WP_002380753.1	primase C-terminal domain-containing protein	-
IU206_RS13470	1891..2130	-	240	WP_002380754.1	helix-turn-helix domain-containing protein	-
IU206_RS13475	2374..2547	-	174	WP_171902975.1	rRNA adenine methyltransferase	-
IU206_RS13480	2492..3229	-	738	WP_194967082.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
IU206_RS13485	3354..3449	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
IU206_RS13490	3498..3581	-	84	Protein_5	peptide-binding protein	-
IU206_RS13495	4125..4988	-	864	WP_002401483.1	zeta toxin family protein	Toxin
IU206_RS13500	4990..5262	-	273	WP_000301765.1	antitoxin	Antitoxin
IU206_RS13505	5279..5488	-	210	WP_000527317.1	peptide-binding protein	-
IU206_RS13510	5565..6461	-	897	WP_118215883.1	ParA family protein	-
IU206_RS13515	6564..8708	-	2145	WP_130006275.1	type IA DNA topoisomerase	-
IU206_RS13520	9029..9244	-	216	WP_076720021.1	helix-turn-helix domain containing protein	-
IU206_RS13525	9222..9815	-	594	WP_171970647.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B)	-	1..196063	196063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T180862 WP_002401483.1 NZ_CP064344:c4988-4125 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T180862 NZ_CP085763:c29599-29312 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT180862 WP_000301765.1 NZ_CP064344:c5262-4990 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT180862 NZ_CP085763:c29838-29599 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3