Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 481958..482529 Replicon chromosome
Accession NZ_CP064343
Organism Enterococcus faecium strain VRE3363

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag IU206_RS02295 Protein ID WP_002286801.1
Coordinates 482188..482529 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag IU206_RS02290 Protein ID WP_002323011.1
Coordinates 481958..482188 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IU206_RS02260 477091..478422 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
IU206_RS02265 478444..479070 + 627 WP_002286816.1 cysteine hydrolase -
IU206_RS02270 479253..479834 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
IU206_RS02275 480566..480913 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
IU206_RS02280 480889..481128 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
IU206_RS02285 481333..481671 - 339 WP_002286804.1 hypothetical protein -
IU206_RS02290 481958..482188 + 231 WP_002323011.1 hypothetical protein Antitoxin
IU206_RS02295 482188..482529 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IU206_RS02300 483379..483564 + 186 WP_002304207.1 hypothetical protein -
IU206_RS02305 484069..484263 + 195 WP_002297028.1 hypothetical protein -
IU206_RS02310 484421..484703 + 283 Protein_458 transposase -
IU206_RS02315 484947..485777 - 831 WP_002286789.1 manganese catalase family protein -
IU206_RS02320 485985..487319 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T180861 WP_002286801.1 NZ_CP064343:482188-482529 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T180861 NZ_CP085757:c3927765-3927547 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT180861 WP_002323011.1 NZ_CP064343:481958-482188 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT180861 NZ_CP085757:c3928167-3927793 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References