Detailed information of TA system TA180856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1405051..1405656
• Replicon: chromosome
• Accession: NZ_CP064334
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: IUD20_RS06390
• Protein ID: WP_004089252.1
• Coordinates: 1405051..1405347 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IUD20_RS06395
• Protein ID: WP_004089254.1
• Coordinates: 1405351..1405656 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD20_RS06365	1400216..1401193	+	978	WP_004087353.1	hypothetical protein	-
IUD20_RS06370	1401452..1401898	+	447	WP_004087355.1	hypothetical protein	-
IUD20_RS06375	1402002..1403246	-	1245	WP_011097983.1	phage tail protein	-
IUD20_RS06380	1403250..1403810	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
IUD20_RS06385	1403807..1404952	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
IUD20_RS06390	1405051..1405347	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD20_RS06395	1405351..1405656	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
IUD20_RS06400	1405667..1406020	-	354	WP_011097986.1	hypothetical protein	-
IUD20_RS06405	1406017..1406658	-	642	WP_004090764.1	hypothetical protein	-
IUD20_RS06410	1406655..1407485	-	831	WP_004090766.1	hypothetical protein	-
IUD20_RS06415	1407482..1407799	-	318	WP_011097873.1	hypothetical protein	-
IUD20_RS06420	1407799..1408569	-	771	WP_004090769.1	hypothetical protein	-
IUD20_RS06425	1408680..1408907	+	228	WP_004091377.1	hypothetical protein	-
IUD20_RS06430	1408891..1409157	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1402002..1444916	42914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T180856 WP_004089252.1 NZ_CP064334:1405051-1405347 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T180856 NZ_CP085757:1748143-1748442 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TATATGAAGCATTGAGTCCTACGTTGAAGGGATACTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT180856 WP_004089254.1 NZ_CP064334:1405351-1405656 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT180856 NZ_CP085757:1748453-1748800 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGGTGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAACGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCGGACGTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A060HBK9