Detailed information of TA system TA180852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsR-graA/MqsR-HigA
• Location: 457618..458283
• Replicon: chromosome
• Accession: NZ_CP064334
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q87EE2
• Locus tag: IUD20_RS01870
• Protein ID: WP_004090696.1
• Coordinates: 457981..458283 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: IUD20_RS01865
• Protein ID: WP_004085172.1
• Coordinates: 457618..457893 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD20_RS01835	453749..454468	-	720	Protein_343	phage late control D family protein	-
IUD20_RS01840	454368..455339	-	972	WP_011097609.1	hypothetical protein	-
IUD20_RS01845	455347..455904	-	558	WP_011097610.1	phage tail protein I	-
IUD20_RS01850	455897..456790	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
IUD20_RS01855	456790..457128	-	339	WP_004090694.1	GPW/gp25 family protein	-
IUD20_RS01860	457326..457607	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD20_RS01865	457618..457893	+	276	WP_004085172.1	HigA family addiction module antidote protein	Antitoxin
IUD20_RS01870	457981..458283	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	Toxin
IUD20_RS01875	458286..458687	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	-
IUD20_RS01880	458756..459343	-	588	WP_011097612.1	phage baseplate assembly protein V	-
IUD20_RS01885	459340..459882	-	543	WP_011097613.1	hypothetical protein	-
IUD20_RS01890	459858..460379	-	522	WP_011097614.1	hypothetical protein	-
IUD20_RS01895	460376..460699	-	324	WP_011097935.1	hypothetical protein	-
IUD20_RS01900	460699..460959	-	261	WP_011097616.1	hypothetical protein	-
IUD20_RS01905	460977..462851	-	1875	WP_011097617.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	445924..469838	23914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10948.71 Da
• Isoelectric Point: 8.0251

>T180852 WP_004090696.1 NZ_CP064334:457981-458283 [Xylella fastidiosa subsp. fastidiosa]
MEKGTSHYKLPVVKALVKAGKVRATATAYQGARELGIKDLTGMCTVVLALTSADFYKSMTTYADHTVFQDVYHAKTASGD
EVYLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 303 bp

>T180852 NZ_CP085755:93937-95262 [Enterobacter hormaechei]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT180852 WP_004085172.1 NZ_CP064334:457618-457893 [Xylella fastidiosa subsp. fastidiosa]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT180852 NZ_CP085755:93639-93932 [Enterobacter hormaechei]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Toxin

• AlphaFold DB: Q87EE2