Detailed information of TA system TA180847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1405641..1406246
• Replicon: chromosome
• Accession: NZ_CP064332
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-11

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: IUD21_RS06395
• Protein ID: WP_004089252.1
• Coordinates: 1405641..1405937 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IUD21_RS06400
• Protein ID: WP_004089254.1
• Coordinates: 1405941..1406246 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD21_RS06370	1400806..1401783	+	978	WP_004087353.1	hypothetical protein	-
IUD21_RS06375	1402042..1402488	+	447	WP_004087355.1	hypothetical protein	-
IUD21_RS06380	1402592..1403836	-	1245	WP_011097983.1	phage tail protein	-
IUD21_RS06385	1403840..1404400	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
IUD21_RS06390	1404397..1405542	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
IUD21_RS06395	1405641..1405937	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD21_RS06400	1405941..1406246	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
IUD21_RS06405	1406257..1406610	-	354	WP_011097986.1	hypothetical protein	-
IUD21_RS06410	1406607..1407248	-	642	WP_004090764.1	hypothetical protein	-
IUD21_RS06415	1407245..1408075	-	831	WP_004090766.1	hypothetical protein	-
IUD21_RS06420	1408072..1408389	-	318	WP_011097873.1	hypothetical protein	-
IUD21_RS06425	1408389..1409159	-	771	WP_004090769.1	hypothetical protein	-
IUD21_RS06430	1409270..1409497	+	228	WP_004091377.1	hypothetical protein	-
IUD21_RS06435	1409481..1409747	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1402592..1445506	42914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T180847 WP_004089252.1 NZ_CP064332:1405641-1405937 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T180847 NZ_CP085753:2915537-2915947 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT180847 WP_004089254.1 NZ_CP064332:1405941-1406246 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT180847 NZ_CP085753:2915290-2915556 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A060HBK9