Detailed information of TA system TA180845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1233484..1234280
• Replicon: chromosome
• Accession: NZ_CP064332
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-11

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: IUD21_RS05450
• Protein ID: WP_004088112.1
• Coordinates: 1233484..1234095 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: IUD21_RS05455
• Protein ID: WP_004088114.1
• Coordinates: 1234092..1234280 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD21_RS05405	1228939..1229826	-	888	WP_012382597.1	YdaU family protein	-
IUD21_RS05410	1229823..1230611	-	789	WP_011097897.1	Bro-N domain-containing protein	-
IUD21_RS05415	1230608..1231240	-	633	WP_004088094.1	Bro-N domain-containing protein	-
IUD21_RS05420	1231237..1231521	-	285	WP_004088096.1	hypothetical protein	-
IUD21_RS05425	1231518..1231766	-	249	WP_011097898.1	hypothetical protein	-
IUD21_RS05430	1231763..1231951	-	189	WP_004088102.1	hypothetical protein	-
IUD21_RS05435	1231979..1232170	-	192	WP_004088104.1	hypothetical protein	-
IUD21_RS05440	1232429..1232674	-	246	WP_004088108.1	hypothetical protein	-
IUD21_RS05445	1232759..1233433	+	675	WP_012382600.1	helix-turn-helix transcriptional regulator	-
IUD21_RS05450	1233484..1234095	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
IUD21_RS05455	1234092..1234280	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
IUD21_RS05460	1234672..1235208	+	537	WP_012382601.1	hypothetical protein	-
IUD21_RS05465	1235205..1235618	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
IUD21_RS05470	1235615..1236016	+	402	WP_004088054.1	hypothetical protein	-
IUD21_RS05475	1236013..1236162	+	150	WP_012382602.1	hypothetical protein	-
IUD21_RS05480	1236248..1236526	+	279	WP_004088050.1	hypothetical protein	-
IUD21_RS05485	1236549..1236740	+	192	WP_012382603.1	hypothetical protein	-
IUD21_RS05490	1236761..1237105	+	345	WP_004088346.1	hypothetical protein	-
IUD21_RS05495	1237145..1237966	+	822	WP_004088345.1	DUF2303 family protein	-
IUD21_RS05500	1237982..1238908	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T180845 WP_004088112.1 NZ_CP064332:c1234095-1233484 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T180845 NZ_CP085753:1889259-1889744 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT180845 WP_004088114.1 NZ_CP064332:c1234280-1234092 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT180845 NZ_CP085753:1889005-1889271 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551