Detailed information of TA system TA180838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1388401..1389006
• Replicon: chromosome
• Accession: NZ_CP064330
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-13

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: IUD22_RS06250
• Protein ID: WP_004089252.1
• Coordinates: 1388401..1388697 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IUD22_RS06255
• Protein ID: WP_004089254.1
• Coordinates: 1388701..1389006 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD22_RS06225	1383566..1384543	+	978	WP_004087353.1	hypothetical protein	-
IUD22_RS06230	1384802..1385248	+	447	WP_004087355.1	hypothetical protein	-
IUD22_RS06235	1385352..1386596	-	1245	WP_011097983.1	phage tail protein	-
IUD22_RS06240	1386600..1387160	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
IUD22_RS06245	1387157..1388302	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
IUD22_RS06250	1388401..1388697	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD22_RS06255	1388701..1389006	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
IUD22_RS06260	1389017..1389370	-	354	WP_011097986.1	hypothetical protein	-
IUD22_RS06265	1389367..1390008	-	642	WP_004090764.1	hypothetical protein	-
IUD22_RS06270	1390005..1390835	-	831	WP_004090766.1	hypothetical protein	-
IUD22_RS06275	1390832..1391149	-	318	WP_011097873.1	hypothetical protein	-
IUD22_RS06280	1391149..1391919	-	771	WP_004090769.1	hypothetical protein	-
IUD22_RS06285	1392030..1392257	+	228	WP_004091377.1	hypothetical protein	-
IUD22_RS06290	1392241..1392507	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1385352..1403651	18299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T180838 WP_004089252.1 NZ_CP064330:1388401-1388697 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T180838 NZ_CP085751:c14638-14153 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT180838 WP_004089254.1 NZ_CP064330:1388701-1389006 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT180838 NZ_CP085751:c14892-14626 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9