Detailed information of TA system TA180836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1216246..1217042
• Replicon: chromosome
• Accession: NZ_CP064330
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-13

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: IUD22_RS05315
• Protein ID: WP_004088112.1
• Coordinates: 1216246..1216857 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: IUD22_RS05320
• Protein ID: WP_004088114.1
• Coordinates: 1216854..1217042 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD22_RS05270	1211701..1212588	-	888	WP_012382597.1	YdaU family protein	-
IUD22_RS05275	1212585..1213373	-	789	WP_011097897.1	Bro-N domain-containing protein	-
IUD22_RS05280	1213370..1214002	-	633	WP_004088094.1	Bro-N domain-containing protein	-
IUD22_RS05285	1213999..1214283	-	285	WP_004088096.1	hypothetical protein	-
IUD22_RS05290	1214280..1214528	-	249	WP_011097898.1	hypothetical protein	-
IUD22_RS05295	1214525..1214713	-	189	WP_004088102.1	hypothetical protein	-
IUD22_RS05300	1214741..1214932	-	192	WP_004088104.1	hypothetical protein	-
IUD22_RS05305	1215191..1215436	-	246	WP_004088108.1	hypothetical protein	-
IUD22_RS05310	1215521..1216195	+	675	WP_012382600.1	helix-turn-helix transcriptional regulator	-
IUD22_RS05315	1216246..1216857	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
IUD22_RS05320	1216854..1217042	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
IUD22_RS05325	1217434..1217970	+	537	WP_012382601.1	hypothetical protein	-
IUD22_RS05330	1217967..1218380	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
IUD22_RS05335	1218377..1218778	+	402	WP_004088054.1	hypothetical protein	-
IUD22_RS05340	1218775..1218924	+	150	WP_012382602.1	hypothetical protein	-
IUD22_RS05345	1219010..1219288	+	279	WP_004088050.1	hypothetical protein	-
IUD22_RS05350	1219311..1219502	+	192	WP_012382603.1	hypothetical protein	-
IUD22_RS05355	1219523..1219867	+	345	WP_004088346.1	hypothetical protein	-
IUD22_RS05360	1219907..1220728	+	822	WP_004088345.1	DUF2303 family protein	-
IUD22_RS05365	1220744..1221670	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1203343..1226792	23449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T180836 WP_004088112.1 NZ_CP064330:c1216857-1216246 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T180836 NZ_CP085748:4263550-4263768 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACTCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT180836 WP_004088114.1 NZ_CP064330:c1217042-1216854 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT180836 NZ_CP085748:4263148-4263522 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551