Detailed information of TA system TA180829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1388137..1388742
• Replicon: chromosome
• Accession: NZ_CP064328
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: IUD25_RS06245
• Protein ID: WP_004089252.1
• Coordinates: 1388137..1388433 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IUD25_RS06250
• Protein ID: WP_004089254.1
• Coordinates: 1388437..1388742 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD25_RS06220	1383302..1384279	+	978	WP_004087353.1	hypothetical protein	-
IUD25_RS06225	1384538..1384984	+	447	WP_004087355.1	hypothetical protein	-
IUD25_RS06230	1385088..1386332	-	1245	WP_011097983.1	phage tail protein	-
IUD25_RS06235	1386336..1386896	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
IUD25_RS06240	1386893..1388038	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
IUD25_RS06245	1388137..1388433	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD25_RS06250	1388437..1388742	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
IUD25_RS06255	1388753..1389106	-	354	WP_011097986.1	hypothetical protein	-
IUD25_RS06260	1389103..1389744	-	642	WP_004090764.1	hypothetical protein	-
IUD25_RS06265	1389741..1390571	-	831	WP_004090766.1	hypothetical protein	-
IUD25_RS06270	1390568..1390885	-	318	WP_011097873.1	hypothetical protein	-
IUD25_RS06275	1390885..1391655	-	771	WP_004090769.1	hypothetical protein	-
IUD25_RS06280	1391766..1391993	+	228	WP_004091377.1	hypothetical protein	-
IUD25_RS06285	1391977..1392243	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD25_RS06290	1392254..1393648	-	1395	WP_194957424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1385088..1403386	18298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T180829 WP_004089252.1 NZ_CP064328:1388137-1388433 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T180829 NZ_CP085748:1378112-1378522 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT180829 WP_004089254.1 NZ_CP064328:1388437-1388742 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT180829 NZ_CP085748:1377865-1378131 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A060HBK9