Detailed information of TA system TA180827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1215982..1216778
• Replicon: chromosome
• Accession: NZ_CP064328
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: IUD25_RS05300
• Protein ID: WP_004088112.1
• Coordinates: 1215982..1216593 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: IUD25_RS05305
• Protein ID: WP_004088114.1
• Coordinates: 1216590..1216778 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD25_RS05255	1211437..1212324	-	888	WP_012382597.1	YdaU family protein	-
IUD25_RS05260	1212321..1213109	-	789	WP_011097897.1	Bro-N domain-containing protein	-
IUD25_RS05265	1213106..1213738	-	633	WP_004088094.1	Bro-N domain-containing protein	-
IUD25_RS05270	1213735..1214019	-	285	WP_004088096.1	hypothetical protein	-
IUD25_RS05275	1214016..1214264	-	249	WP_011097898.1	hypothetical protein	-
IUD25_RS05280	1214261..1214449	-	189	WP_004088102.1	hypothetical protein	-
IUD25_RS05285	1214477..1214668	-	192	WP_004088104.1	hypothetical protein	-
IUD25_RS05290	1214927..1215172	-	246	WP_004088108.1	hypothetical protein	-
IUD25_RS05295	1215257..1215931	+	675	WP_012382600.1	helix-turn-helix transcriptional regulator	-
IUD25_RS05300	1215982..1216593	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
IUD25_RS05305	1216590..1216778	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
IUD25_RS05310	1217170..1217706	+	537	WP_012382601.1	hypothetical protein	-
IUD25_RS05315	1217703..1218116	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
IUD25_RS05320	1218113..1218514	+	402	WP_004088054.1	hypothetical protein	-
IUD25_RS05325	1218511..1218660	+	150	WP_012382602.1	hypothetical protein	-
IUD25_RS05330	1218746..1219024	+	279	WP_004088050.1	hypothetical protein	-
IUD25_RS05335	1219047..1219238	+	192	WP_012382603.1	hypothetical protein	-
IUD25_RS05340	1219259..1219603	+	345	WP_004088346.1	hypothetical protein	-
IUD25_RS05345	1219643..1220464	+	822	WP_004088345.1	DUF2303 family protein	-
IUD25_RS05350	1220480..1221406	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1203079..1226528	23449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T180827 WP_004088112.1 NZ_CP064328:c1216593-1215982 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T180827 NZ_CP085748:132050-132337 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT180827 WP_004088114.1 NZ_CP064328:c1216778-1216590 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT180827 NZ_CP085748:131741-132043 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551