Detailed information of TA system TA180820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1361449..1362041
• Replicon: chromosome
• Accession: NZ_CP064326
• Organism: Xylella fastidiosa subsp. multiplex strain Riv5

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: IUD19_RS06025
• Protein ID: WP_004085590.1
• Coordinates: 1361449..1361745 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IUD19_RS06030
• Protein ID: WP_004085592.1
• Coordinates: 1361748..1362041 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD19_RS06000	1356619..1357587	+	969	WP_004084992.1	hypothetical protein	-
IUD19_RS06005	1357846..1358292	+	447	WP_004084994.1	hypothetical protein	-
IUD19_RS06010	1358396..1359640	-	1245	WP_194957572.1	phage tail protein	-
IUD19_RS06015	1359644..1360204	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
IUD19_RS06020	1360201..1361346	-	1146	WP_194957573.1	baseplate J/gp47 family protein	-
IUD19_RS06025	1361449..1361745	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD19_RS06030	1361748..1362041	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
IUD19_RS06035	1362097..1362450	-	354	WP_004084998.1	hypothetical protein	-
IUD19_RS06040	1362447..1363088	-	642	WP_140189635.1	hypothetical protein	-
IUD19_RS06045	1363085..1363915	-	831	WP_004085594.1	hypothetical protein	-
IUD19_RS06050	1363912..1364229	-	318	WP_169710077.1	hypothetical protein	-
IUD19_RS06055	1364229..1364999	-	771	WP_169710321.1	hypothetical protein	-
IUD19_RS06060	1365110..1365337	+	228	WP_004091377.1	hypothetical protein	-
IUD19_RS06065	1365321..1365587	+	267	WP_004087087.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1355747..1389767	34020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T180820 WP_004085590.1 NZ_CP064326:1361449-1361745 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T180820 NZ_CP085746:19927-20337 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT180820 WP_004085592.1 NZ_CP064326:1361748-1362041 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT180820 NZ_CP085746:19700-19930 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8