Detailed information of TA system TA180813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1239999..1240795
• Replicon: chromosome
• Accession: NZ_CP064325
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: -
• Locus tag: IUD24_RS05425
• Protein ID: WP_004087040.1
• Coordinates: 1239999..1240610 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: A0A060HF63
• Locus tag: IUD24_RS05430
• Protein ID: WP_004087046.1
• Coordinates: 1240607..1240795 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD24_RS05400	1237294..1237875	-	582	WP_012337852.1	Bro-N domain-containing protein	-
IUD24_RS05405	1238135..1238518	-	384	WP_012337854.1	hypothetical protein	-
IUD24_RS05410	1238606..1238935	-	330	WP_021358651.1	hypothetical protein	-
IUD24_RS05415	1238925..1239170	-	246	WP_012337855.1	hypothetical protein	-
IUD24_RS05420	1239169..1239948	+	780	WP_021358652.1	helix-turn-helix transcriptional regulator	-
IUD24_RS05425	1239999..1240610	-	612	WP_004087040.1	Fic/DOC family protein	Toxin
IUD24_RS05430	1240607..1240795	-	189	WP_004087046.1	antitoxin VbhA family protein	Antitoxin
IUD24_RS05435	1241176..1241580	+	405	WP_012337857.1	hypothetical protein	-
IUD24_RS05440	1241577..1242038	+	462	WP_012337858.1	DUF1566 domain-containing protein	-
IUD24_RS05445	1242035..1242442	+	408	WP_012337859.1	hypothetical protein	-
IUD24_RS05450	1242439..1242630	+	192	WP_004086069.1	hypothetical protein	-
IUD24_RS05455	1242662..1243231	-	570	WP_012337860.1	DEAD/DEAH box helicase	-
IUD24_RS05460	1243316..1243720	+	405	WP_004085029.1	hypothetical protein	-
IUD24_RS05465	1243722..1244000	-	279	WP_012337663.1	VRR-NUC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1212106..1263051	50945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22961.76 Da
• Isoelectric Point: 5.1271

>T180813 WP_004087040.1 NZ_CP064325:c1240610-1239999 [Xylella fastidiosa subsp. multiplex]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGWLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T180813 NZ_CP085743:1649616-1649834 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6855.80 Da
• Isoelectric Point: 4.9859

>AT180813 WP_004087046.1 NZ_CP064325:c1240795-1240607 [Xylella fastidiosa subsp. multiplex]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQMA

Nucleotide

• Length: 189 bp

>AT180813 NZ_CP085743:1649214-1649588 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A060HF63