Detailed information of TA system TA180810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1025951..1026705
• Replicon: chromosome
• Accession: NZ_CP064325
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IUD24_RS04395
• Protein ID: WP_004085397.1
• Coordinates: 1025951..1026436 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: IUD24_RS04400
• Protein ID: WP_004085396.1
• Coordinates: 1026433..1026705 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD24_RS04385	1022804..1023610	+	807	WP_012337796.1	oxidoreductase	-
IUD24_RS04390	1023750..1025615	+	1866	WP_012337797.1	toprim domain-containing protein	-
IUD24_RS04395	1025951..1026436	-	486	WP_004085397.1	GNAT family N-acetyltransferase	Toxin
IUD24_RS04400	1026433..1026705	-	273	WP_004085396.1	DUF1778 domain-containing protein	Antitoxin
IUD24_RS04405	1027057..1027962	-	906	WP_004085395.1	AEC family transporter	-
IUD24_RS04410	1028078..1028696	+	619	Protein_852	IS607 family transposase	-
IUD24_RS04415	1029164..1030957	+	1794	WP_031345943.1	glycosyl hydrolase family 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (35-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-85)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17858.66 Da
• Isoelectric Point: 9.9283

>T180810 WP_004085397.1 NZ_CP064325:c1026436-1025951 [Xylella fastidiosa subsp. multiplex]
VNMQISTPHSLTAAHRLDEFNCGEPSLDDWLKRRALTNHLNGASRTFVVIDANQYVLGYYALAAGAVAHQEATRAIRRNM
PDPVPVMVLGRLAVDTRAQGIKLGAALLKDTVTRVQSIAENAGVRALVVHALNERAKQFYEYYGFRASPMHLMTLMLPIK
F

Nucleotide

• Length: 486 bp

>T180810 NZ_CP085742:39233-39538 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10264.77 Da
• Isoelectric Point: 5.0488

>AT180810 WP_004085396.1 NZ_CP064325:c1026705-1026433 [Xylella fastidiosa subsp. multiplex]
MRDASINLRAMPEQRDLIDQAANLLGKNRSDFMLEAACERAKAIILDQVFFNLDEERFRQFTVLLNAPQDTNPGLNRLMA
IKAPWSISNT

Nucleotide

• Length: 273 bp

>AT180810 NZ_CP085742:39013-39231 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA