Detailed information of TA system TA180809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 472298..472884
• Replicon: chromosome
• Accession: NZ_CP064325
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q87BV5
• Locus tag: IUD24_RS01960
• Protein ID: WP_004089517.1
• Coordinates: 472630..472884 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: IUD24_RS01955
• Protein ID: WP_004085025.1
• Coordinates: 472298..472633 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD24_RS01925	469072..469647	-	576	WP_012337655.1	terminase small subunit	-
IUD24_RS01930	469778..470002	-	225	WP_012337656.1	hypothetical protein	-
IUD24_RS01935	470004..470465	-	462	WP_012337657.1	hypothetical protein	-
IUD24_RS01940	470455..470784	-	330	WP_012337658.1	phage holin family protein	-
IUD24_RS01945	470777..471277	-	501	WP_012337659.1	lysozyme	-
IUD24_RS01950	471377..472108	-	732	WP_012337660.1	site-specific DNA-methyltransferase	-
IUD24_RS01955	472298..472633	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IUD24_RS01960	472630..472884	-	255	WP_004089517.1	type II toxin-antitoxin system HicA family toxin	Toxin
IUD24_RS01965	472937..473659	-	723	WP_012337661.1	hypothetical protein	-
IUD24_RS01970	473602..473949	-	348	WP_162010044.1	hypothetical protein	-
IUD24_RS01975	474197..475405	+	1209	Protein_371	DNA polymerase	-
IUD24_RS01980	475402..475680	+	279	WP_012337663.1	VRR-NUC domain-containing protein	-
IUD24_RS01985	475682..475987	-	306	WP_012337664.1	hypothetical protein	-
IUD24_RS01990	476072..477490	+	1419	WP_012337665.1	DEAD/DEAH box helicase	-
IUD24_RS01995	477487..477732	+	246	WP_012337666.1	DUF4224 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446878..481250	34372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9639.14 Da
• Isoelectric Point: 11.2766

>T180809 WP_004089517.1 NZ_CP064325:c472884-472630 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T180809 NZ_CP085742:c38223-37807 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT180809 WP_004085025.1 NZ_CP064325:c472633-472298 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT180809 NZ_CP085742:c38450-38220 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q87BV5

Antitoxin

• AlphaFold DB: A0A060HAK5