Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2678023..2678674 Replicon chromosome
Accession NZ_CP064316
Organism Lacticaseibacillus paracasei subsp. tolerans strain MGB0761

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LacP0761_RS12920 Protein ID WP_003567661.1
Coordinates 2678023..2678406 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LacP0761_RS12925 Protein ID WP_003567665.1
Coordinates 2678426..2678674 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LacP0761_RS12915 2677318..2677839 - 522 WP_003567657.1 QueT transporter family protein -
LacP0761_RS12920 2678023..2678406 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LacP0761_RS12925 2678426..2678674 - 249 WP_003567665.1 antitoxin Antitoxin
LacP0761_RS12930 2678742..2679878 - 1137 WP_003581082.1 alanine racemase -
LacP0761_RS12935 2679865..2680239 - 375 WP_003567669.1 holo-ACP synthase -
LacP0761_RS12940 2680408..2681916 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
LacP0761_RS12945 2682042..2682185 - 144 WP_016380391.1 hypothetical protein -
LacP0761_RS12950 2682216..2683604 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T180806 WP_003567661.1 NZ_CP064316:c2678406-2678023 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T180806 NZ_CP085740:c4812101-4811817 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT180806 WP_003567665.1 NZ_CP064316:c2678674-2678426 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT180806 NZ_CP085740:c4812333-4812091 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References