180802

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2761521..2762172 Replicon chromosome
Accession NZ_CP064311
Organism Lacticaseibacillus paracasei subsp. tolerans strain MGB0625

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag LacP0625_RS13420 Protein ID WP_194957911.1
Coordinates 2761521..2761904 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LacP0625_RS13425 Protein ID WP_003567665.1
Coordinates 2761924..2762172 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LacP0625_RS13415 2760816..2761337 - 522 WP_003567657.1 QueT transporter family protein -
LacP0625_RS13420 2761521..2761904 - 384 WP_194957911.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LacP0625_RS13425 2761924..2762172 - 249 WP_003567665.1 antitoxin Antitoxin
LacP0625_RS13430 2762240..2763376 - 1137 WP_016369534.1 alanine racemase -
LacP0625_RS13435 2763363..2763737 - 375 WP_003567669.1 holo-ACP synthase -
LacP0625_RS13440 2763907..2765415 - 1509 WP_016387847.1 DEAD/DEAH box helicase -
LacP0625_RS13445 2765541..2765684 - 144 WP_003588978.1 hypothetical protein -
LacP0625_RS13450 2765715..2767103 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2755977..2874006 118029


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13831.07 Da        Isoelectric Point: 10.8262

>T180802 WP_194957911.1 NZ_CP064311:c2761904-2761521 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVDLVSLPKPKTYNKN

Download         Length: 384 bp

>T180802 NZ_CP085740:2020213-2020315 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT180802 WP_003567665.1 NZ_CP064311:c2762172-2761924 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT180802 NZ_CP085740:c2020321-2020177 [Klebsiella pneumoniae]
ACATATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References