Detailed information of TA system TA180800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 510992..511620
• Replicon: chromosome
• Accession: NZ_CP064311
• Organism: Lacticaseibacillus paracasei subsp. tolerans strain MGB0625

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S2NVS0
• Locus tag: LacP0625_RS02325
• Protein ID: WP_016373562.1
• Coordinates: 511252..511620 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: LacP0625_RS02320
• Protein ID: WP_003583264.1
• Coordinates: 510992..511252 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LacP0625_RS02290	506782..507312	+	531	WP_012490988.1	DNA-directed RNA polymerase subunit sigma-24	-
LacP0625_RS02295	507480..507632	+	153	WP_003563363.1	YvrJ family protein	-
LacP0625_RS02300	507642..508406	+	765	WP_016387929.1	LysM peptidoglycan-binding domain-containing protein	-
LacP0625_RS02305	508617..509120	+	504	WP_003563372.1	TetR/AcrR family transcriptional regulator	-
LacP0625_RS02310	509125..510186	+	1062	WP_016387927.1	ABC transporter permease	-
LacP0625_RS02315	510191..510880	+	690	WP_016387926.1	ABC transporter ATP-binding protein	-
LacP0625_RS02320	510992..511252	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
LacP0625_RS02325	511252..511620	-	369	WP_016373562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LacP0625_RS02330	511912..513282	-	1371	WP_016387925.1	FAD-dependent oxidoreductase	-
LacP0625_RS02335	513646..514464	-	819	WP_019914724.1	Cof-type HAD-IIB family hydrolase	-
LacP0625_RS02340	514781..515050	-	270	WP_003563382.1	type II toxin-antitoxin system YafQ family toxin	-
LacP0625_RS02345	515291..516298	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14923.01 Da
• Isoelectric Point: 8.3986

>T180800 WP_016373562.1 NZ_CP064311:c511620-511252 [Lacticaseibacillus paracasei subsp. tolerans]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMPERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQANLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T180800 NZ_CP085740:693340-693825 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT180800 WP_003583264.1 NZ_CP064311:c511252-510992 [Lacticaseibacillus paracasei subsp. tolerans]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT180800 NZ_CP085740:693050-693343 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S2NVS0