Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1780289..1780939 Replicon chromosome
Accession NZ_CP064303
Organism Lacticaseibacillus casei strain MGB0470

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LacC0470_RS08365 Protein ID WP_005686631.1
Coordinates 1780289..1780672 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A5R8LDU7
Locus tag LacC0470_RS08370 Protein ID WP_025013377.1
Coordinates 1780691..1780939 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LacC0470_RS08345 1775633..1776640 + 1008 WP_003662706.1 L-lactate dehydrogenase -
LacC0470_RS08350 1776948..1778318 + 1371 WP_025013379.1 peptidoglycan binding domain-containing protein -
LacC0470_RS08355 1778533..1779198 + 666 WP_010491258.1 CBS domain-containing protein -
LacC0470_RS08360 1779521..1780219 + 699 WP_025013378.1 L,D-transpeptidase -
LacC0470_RS08365 1780289..1780672 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LacC0470_RS08370 1780691..1780939 - 249 WP_025013377.1 antitoxin Antitoxin
LacC0470_RS08375 1781038..1782177 - 1140 WP_025013376.1 alanine racemase -
LacC0470_RS08380 1782164..1782538 - 375 WP_039639939.1 holo-ACP synthase -
LacC0470_RS08385 1782725..1783777 - 1053 WP_039638840.1 IS30 family transposase -
LacC0470_RS08390 1784013..1785524 - 1512 WP_025013796.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T180796 WP_005686631.1 NZ_CP064303:c1780672-1780289 [Lacticaseibacillus casei]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T180796 NZ_CP085732:c125194-124883 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8999.12 Da        Isoelectric Point: 4.6061

>AT180796 WP_025013377.1 NZ_CP064303:c1780939-1780691 [Lacticaseibacillus casei]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVVMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT180796 NZ_CP085732:c124886-124467 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A5R8LDU7

References