Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1708505..1709156 Replicon chromosome
Accession NZ_CP064299
Organism Lacticaseibacillus paracasei subsp. tolerans strain MGB0245

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LacP0245_RS08260 Protein ID WP_003567661.1
Coordinates 1708505..1708888 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LacP0245_RS08265 Protein ID WP_003567665.1
Coordinates 1708908..1709156 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LacP0245_RS08255 1707800..1708321 - 522 WP_003567657.1 QueT transporter family protein -
LacP0245_RS08260 1708505..1708888 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LacP0245_RS08265 1708908..1709156 - 249 WP_003567665.1 antitoxin Antitoxin
LacP0245_RS08270 1709224..1710360 - 1137 WP_016369534.1 alanine racemase -
LacP0245_RS08275 1710347..1710721 - 375 WP_003567669.1 holo-ACP synthase -
LacP0245_RS08280 1710891..1712399 - 1509 WP_016387847.1 DEAD/DEAH box helicase -
LacP0245_RS08285 1712525..1712668 - 144 WP_003588978.1 hypothetical protein -
LacP0245_RS08290 1712699..1714087 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T180794 WP_003567661.1 NZ_CP064299:c1708888-1708505 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T180794 NZ_CP085731:168253-168669 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT180794 WP_003567665.1 NZ_CP064299:c1709156-1708908 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT180794 NZ_CP085731:168026-168256 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References