Detailed information of TA system TA180792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2011828..2012464
• Replicon: chromosome
• Accession: NZ_CP064275
• Organism: Priestia megaterium strain B1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: IQ966_RS10775
• Protein ID: WP_013055004.1
• Coordinates: 2012114..2012464 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: IQ966_RS10770
• Protein ID: WP_013055003.1
• Coordinates: 2011828..2012109 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ966_RS10745	2007194..2008165	+	972	WP_115652913.1	UV DNA damage repair endonuclease UvsE	-
IQ966_RS10750	2008174..2008776	-	603	WP_014461974.1	rhomboid family intramembrane serine protease	-
IQ966_RS10755	2008841..2009206	+	366	WP_115655114.1	holo-ACP synthase	-
IQ966_RS10760	2009319..2010323	+	1005	WP_274596470.1	outer membrane lipoprotein carrier protein LolA	-
IQ966_RS10765	2010437..2011627	+	1191	WP_115652915.1	alanine racemase	-
IQ966_RS10770	2011828..2012109	+	282	WP_013055003.1	hypothetical protein	Antitoxin
IQ966_RS10775	2012114..2012464	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IQ966_RS10780	2012622..2013458	+	837	WP_014461969.1	RsbT co-antagonist protein RsbRA	-
IQ966_RS10785	2013461..2013817	+	357	WP_013055006.1	STAS domain-containing protein	-
IQ966_RS10790	2013821..2014222	+	402	WP_013055007.1	anti-sigma regulatory factor	-
IQ966_RS10795	2014236..2015246	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
IQ966_RS10800	2015306..2015638	+	333	WP_014461968.1	anti-sigma factor antagonist	-
IQ966_RS10805	2015635..2016120	+	486	WP_014461967.1	anti-sigma B factor RsbW	-
IQ966_RS10810	2016086..2016880	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T180792 WP_013055004.1 NZ_CP064275:2012114-2012464 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T180792 NZ_CP085729:c4626650-4626432 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT180792 WP_013055003.1 NZ_CP064275:2011828-2012109 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT180792 NZ_CP085729:c4627051-4626677 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9