Detailed information of TA system TA180791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 3094256..3094892
• Replicon: chromosome
• Accession: NZ_CP064270
• Organism: Priestia megaterium strain N447-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: IQ863_RS15875
• Protein ID: WP_013055004.1
• Coordinates: 3094256..3094606 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: IQ863_RS15880
• Protein ID: WP_013055003.1
• Coordinates: 3094611..3094892 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ863_RS15840	3089840..3090634	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
IQ863_RS15845	3090600..3091085	-	486	WP_014461967.1	anti-sigma B factor RsbW	-
IQ863_RS15850	3091082..3091414	-	333	WP_014461968.1	anti-sigma factor antagonist	-
IQ863_RS15855	3091474..3092484	-	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
IQ863_RS15860	3092498..3092899	-	402	WP_013055007.1	anti-sigma regulatory factor	-
IQ863_RS15865	3092903..3093259	-	357	WP_013055006.1	STAS domain-containing protein	-
IQ863_RS15870	3093262..3094098	-	837	WP_014461969.1	RsbT co-antagonist protein RsbRA	-
IQ863_RS15875	3094256..3094606	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IQ863_RS15880	3094611..3094892	-	282	WP_013055003.1	hypothetical protein	Antitoxin
IQ863_RS15885	3095093..3096283	-	1191	WP_014461971.1	alanine racemase	-
IQ863_RS15890	3096397..3097401	-	1005	WP_279400964.1	outer membrane lipoprotein carrier protein LolA	-
IQ863_RS15895	3097514..3097879	-	366	WP_042992604.1	holo-ACP synthase	-
IQ863_RS15900	3097944..3098546	+	603	WP_014461974.1	rhomboid family intramembrane serine protease	-
IQ863_RS15905	3098555..3099526	-	972	WP_042992385.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T180791 WP_013055004.1 NZ_CP064270:c3094606-3094256 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T180791 NZ_CP085729:4016849-4017382 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT180791 WP_013055003.1 NZ_CP064270:c3094892-3094611 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT180791 NZ_CP085729:4016572-4016838 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9