Detailed information of TA system TA180786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 44128..44687
• Replicon: plasmid pJX10-2
• Accession: NZ_CP064260
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: IS497_RS29355
• Protein ID: WP_000421257.1
• Coordinates: 44412..44687 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: IS497_RS29350
• Protein ID: WP_001178089.1
• Coordinates: 44128..44412 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS497_RS29325	39336..39890	-	555	WP_032488579.1	aminoglycoside N-acetyltransferase AAC(6')-Ib3	-
IS497_RS29330	40070..41083	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
IS497_RS29335	41286..41522	+	237	Protein_60	DUF3330 domain-containing protein	-
IS497_RS29340	41586..42290	+	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS497_RS29345	42480..43484	+	1005	WP_021546935.1	IS110-like element IS4321 family transposase	-
IS497_RS29350	44128..44412	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
IS497_RS29355	44412..44687	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS497_RS29360	44737..45434	-	698	Protein_65	IS1 family transposase	-
IS497_RS29365	45508..45867	+	360	WP_085452786.1	colicin	-
IS497_RS29370	45889..46224	-	336	WP_001080730.1	colicin 1A immunity protein	-
IS497_RS29375	46353..46700	+	348	WP_000142451.1	hypothetical protein	-
IS497_RS29380	46787..47491	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS497_RS29385	47555..48040	+	486	Protein_70	recombinase family protein	-
IS497_RS29390	48117..49508	+	1392	WP_000085084.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / blaCTX-M-14 / qacE / cmlA1	-	1..90714	90714
	-	inside	IScluster/Tn	blaTEM-1B / blaCTX-M-14 / qacE / cmlA1	-	21304..54789	33485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T180786 WP_000421257.1 NZ_CP064260:44412-44687 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T180786 NZ_CP085729:c2542732-2542322 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT180786 WP_001178089.1 NZ_CP064260:44128-44412 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT180786 NZ_CP085729:c2542979-2542713 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8