Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 381108..381751 Replicon plasmid pJX10-1
Accession NZ_CP064259
Organism Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS497_RS28950 Protein ID WP_001044770.1
Coordinates 381335..381751 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS497_RS28945 Protein ID WP_001261282.1
Coordinates 381108..381338 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS497_RS28910 376432..376737 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
IS497_RS28915 376739..376957 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IS497_RS28920 377516..378442 + 927 WP_001290414.1 hypothetical protein -
IS497_RS28925 378492..378746 + 255 WP_000678528.1 hypothetical protein -
IS497_RS28930 379244..379588 + 345 WP_000792769.1 hypothetical protein -
IS497_RS28935 379762..380763 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS497_RS28940 380912..381151 - 240 Protein_406 hypothetical protein -
IS497_RS28945 381108..381338 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS497_RS28950 381335..381751 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS497_RS28955 381825..383387 + 1563 WP_004206609.1 AAA family ATPase -
IS497_RS28960 383372..384394 + 1023 WP_000361404.1 helicase UvrD -
IS497_RS28965 384650..385347 + 698 Protein_411 IS1 family transposase -
IS497_RS28970 385376..385648 + 273 Protein_412 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA 1..394328 394328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180781 WP_001044770.1 NZ_CP064259:381335-381751 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180781 NZ_CP085714:342783-343133 [Peribacillus asahii]
ATTGTTGTTAAGCGTGGTGACGTATACTTCGCAGATCTTTCTCCAGTTGTCGGCTCAGAACAAGGTGGAACCCGTCCGGT
ACTCATTCTTCAAAATGATATCGGAAATCGCTTTAGTCCGACAGTGATTGTGGCAGCCATTACAGCTCAAATTCAAAAAG
CAAAATTGCCAACACATGTGGAGATTAACGCGAAAAAATATGGATTCGAGCGAGATTCCGTTATTCTTTTAGAACAAATT
CGCACGATTGATAAGCAACGCTTGACAGACAAAATTACGTATCTGGATGAAACAATGATGCAAAAAGTAAACGAAGCTTT
ACAGATTAGTTTAGGGCTAAAAGAATTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180781 WP_001261282.1 NZ_CP064259:381108-381338 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180781 NZ_CP085714:342491-342775 [Peribacillus asahii]
GTGTCTGAATCCGGCGCAACAAGAGAGATAATAATTCGATTACCTCAAAATGTATTAACTGAGTTAGACGGGTATGCGAG
TGAAGAAAACGTTAATCGCAGTGAGTTTATTTATCGTGCCACGACTATGTATCTTCGCAAAAAGAAAGAATTTCGGGAAT
CAATGAAACGTGGCTATATCGAAATGGCGGCCATTAATTTAACAATCGCTTCTGAAGCATTTCAGGCTGAATATCCAGAA
TATGAAGCTGAACATGCTGTCGAGCGATTAGCTAGTGGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References