Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 376432..376957 Replicon plasmid pJX10-1
Accession NZ_CP064259
Organism Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS497_RS28910 Protein ID WP_001159871.1
Coordinates 376432..376737 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS497_RS28915 Protein ID WP_000813634.1
Coordinates 376739..376957 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS497_RS28875 372186..372830 + 645 WP_000633913.1 AAA family ATPase -
IS497_RS28880 372824..373099 + 276 WP_000239529.1 hypothetical protein -
IS497_RS28885 373291..373717 - 427 Protein_395 tyrosine-type recombinase/integrase -
IS497_RS28890 373897..374487 + 591 Protein_396 colicin-B -
IS497_RS28895 374503..375200 - 698 Protein_397 IS1 family transposase -
IS497_RS28900 375256..375450 - 195 Protein_398 colicin M immunity protein -
IS497_RS28905 375622..376431 - 810 WP_000016963.1 site-specific integrase -
IS497_RS28910 376432..376737 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS497_RS28915 376739..376957 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS497_RS28920 377516..378442 + 927 WP_001290414.1 hypothetical protein -
IS497_RS28925 378492..378746 + 255 WP_000678528.1 hypothetical protein -
IS497_RS28930 379244..379588 + 345 WP_000792769.1 hypothetical protein -
IS497_RS28935 379762..380763 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS497_RS28940 380912..381151 - 240 Protein_406 hypothetical protein -
IS497_RS28945 381108..381338 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IS497_RS28950 381335..381751 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA 1..394328 394328
- flank IS/Tn - - 374503..375006 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180780 WP_001159871.1 NZ_CP064259:c376737-376432 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180780 NZ_CP064259:c376737-376432 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180780 WP_000813634.1 NZ_CP064259:c376957-376739 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180780 NZ_CP064259:c376957-376739 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References