Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 341623..342533 Replicon plasmid pJX10-1
Accession NZ_CP064259
Organism Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag IS497_RS28690 Protein ID WP_004026354.1
Coordinates 341623..342093 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag IS497_RS28695 Protein ID WP_004026357.1
Coordinates 342090..342533 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS497_RS28675 337242..338191 + 950 Protein_353 DUF1173 family protein -
IS497_RS28680 338981..339961 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
IS497_RS28685 340777..341474 - 698 Protein_355 IS1 family transposase -
IS497_RS28690 341623..342093 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
IS497_RS28695 342090..342533 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
IS497_RS28700 342634..343137 - 504 Protein_358 DUF4113 domain-containing protein -
IS497_RS28705 343343..344323 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
IS497_RS28710 344389..345696 + 1308 Protein_360 FepA family TonB-dependent siderophore receptor -
IS497_RS28715 345758..345979 + 222 WP_004213615.1 hypothetical protein -
IS497_RS28720 346122..347024 - 903 WP_004213613.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA 1..394328 394328
- inside IScluster/Tn - - 334384..344323 9939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T180775 WP_004026354.1 NZ_CP064259:c342093-341623 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T180775 NZ_CP085704:4314903-4315205 [Pseudomonas sp. HN2-3]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGCATGGACTCCGGGACGACACGGCGCGTATCCGGATTATTGC
TCGTATCAACCGCTTGATGGAGGGCCTGCCTGGCGACGTTTCAGCAGTTGGCCATGGTGTAAGTGAATTGAGAATTCACT
ATGGGCCGGGCTACCGCGTGTACTTTCACCAATCAGGCGATACGTTGGTAATTCTGCTCTGCGGCGGGCACAAAAGCAGC
CAACGGCGGGACATCAAAGCGGCTCACGAAATCCTGCGCTCCTGGAGGATGCAACATGACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT180775 WP_004026357.1 NZ_CP064259:c342533-342090 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT180775 NZ_CP085704:4315198-4315491 [Pseudomonas sp. HN2-3]
ATGACTGAAACATTTTTTGACTTCGATGCGGCTGATTACCTGACCACCCCGCAAGCCATCGCGCAGTTCATGTCCGATGC
GCTTGAAACGGGCGATGCCAGCTACGTGGCCAAGGCCGTTGGTGTGGTAGCACGTGCCAAAGGCATGAGCGAATTGGCGA
AAGAGACGGGTTTGTCACGTGAACAACTTTATCGCTCGTTCAGCGAGCACGGTAATCCGACGTTGAAGTCGTTTCTTGCC
GTCATGAAGGCGCTGGGAATAGACATGACCGCCAAACCTCATCTTGCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References