Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5377736..5378361 Replicon chromosome
Accession NZ_CP064258
Organism Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS497_RS26535 Protein ID WP_002882817.1
Coordinates 5377736..5378119 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS497_RS26540 Protein ID WP_004150355.1
Coordinates 5378119..5378361 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS497_RS26520 5375102..5376004 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS497_RS26525 5376001..5376636 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS497_RS26530 5376633..5377562 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS497_RS26535 5377736..5378119 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS497_RS26540 5378119..5378361 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS497_RS26545 5378566..5379483 + 918 WP_002882812.1 alpha/beta hydrolase -
IS497_RS26550 5379497..5380438 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS497_RS26555 5380483..5380920 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS497_RS26560 5380917..5381777 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS497_RS26565 5381771..5382370 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180772 WP_002882817.1 NZ_CP064258:c5378119-5377736 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180772 NZ_CP085699:4808877-4809188 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180772 WP_004150355.1 NZ_CP064258:c5378361-5378119 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180772 NZ_CP085699:4809185-4809631 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References