Detailed information of TA system TA180753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5232..5791
• Replicon: plasmid pJX1-2
• Accession: NZ_CP064254
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: IS496_RS28115
• Protein ID: WP_000421257.1
• Coordinates: 5516..5791 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: IS496_RS28110
• Protein ID: WP_001178087.1
• Coordinates: 5232..5516 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS496_RS28085	576..818	-	243	WP_021515452.1	PilI type IV pilus biogenesis protein	-
IS496_RS28090	899..1042	-	144	WP_000176500.1	hypothetical protein	-
IS496_RS28095	1309..1971	-	663	WP_005082527.1	hypothetical protein	-
IS496_RS28100	2111..2752	-	642	WP_194813330.1	transcription termination factor NusG	-
IS496_RS28105	3455..4327	+	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
IS496_RS28110	5232..5516	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
IS496_RS28115	5516..5791	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS496_RS28120	5956..6237	+	282	WP_001105066.1	hypothetical protein	-
IS496_RS28125	6428..6694	-	267	WP_000627831.1	hypothetical protein	-
IS496_RS28130	6832..7434	-	603	WP_000521606.1	hypothetical protein	-
IS496_RS28135	7610..8662	-	1053	WP_000655519.1	hypothetical protein	-
IS496_RS28140	8875..9465	-	591	WP_001401589.1	hypothetical protein	-
IS496_RS28145	9465..9719	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-14	-	1..102456	102456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T180753 WP_000421257.1 NZ_CP064254:5516-5791 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T180753 NZ_CP085696:c4212912-4212628 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGACTTATGAACTGGAAATCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT180753 WP_001178087.1 NZ_CP064254:5232-5516 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT180753 NZ_CP085696:c4213144-4212902 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTTTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625