Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11087..11730 Replicon plasmid pJX1-1
Accession NZ_CP064253
Organism Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS496_RS27070 Protein ID WP_001044770.1
Coordinates 11314..11730 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS496_RS27065 Protein ID WP_001261282.1
Coordinates 11087..11317 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS496_RS27030 6310..7089 - 780 WP_013214009.1 site-specific integrase -
IS496_RS27035 7273..8277 - 1005 WP_011977814.1 hypothetical protein -
IS496_RS27040 8307..8510 - 204 WP_011977813.1 hypothetical protein -
IS496_RS27045 8556..9077 - 522 WP_013214008.1 hypothetical protein -
IS496_RS27050 9135..9527 - 393 WP_011977811.1 hypothetical protein -
IS496_RS27055 9564..10508 - 945 WP_011977810.1 hypothetical protein -
IS496_RS27060 10669..11130 - 462 WP_014343465.1 hypothetical protein -
IS496_RS27065 11087..11317 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS496_RS27070 11314..11730 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS496_RS27075 11804..13366 + 1563 WP_004206609.1 AAA family ATPase -
IS496_RS27080 13351..14373 + 1023 WP_000361404.1 helicase UvrD -
IS496_RS27085 14629..15326 + 698 Protein_17 IS1 family transposase -
IS496_RS27090 15355..15627 + 273 Protein_18 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2 - 1..167834 167834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180744 WP_001044770.1 NZ_CP064253:11314-11730 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180744 NZ_CP085696:311031-311399 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTTGCTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180744 WP_001261282.1 NZ_CP064253:11087-11317 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180744 NZ_CP085696:310813-311034 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References