Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5406918..5407543 Replicon chromosome
Accession NZ_CP064252
Organism Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS496_RS26635 Protein ID WP_002882817.1
Coordinates 5406918..5407301 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS496_RS26640 Protein ID WP_004150355.1
Coordinates 5407301..5407543 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS496_RS26620 5404284..5405186 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS496_RS26625 5405183..5405818 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS496_RS26630 5405815..5406744 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS496_RS26635 5406918..5407301 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS496_RS26640 5407301..5407543 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS496_RS26645 5407748..5408665 + 918 WP_002882812.1 alpha/beta hydrolase -
IS496_RS26650 5408679..5409620 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS496_RS26655 5409665..5410102 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS496_RS26660 5410099..5410959 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS496_RS26665 5410953..5411552 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180743 WP_002882817.1 NZ_CP064252:c5407301-5406918 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180743 NZ_CP085696:200943-201428 [Salmonella enterica subsp. enterica serovar Saintpaul]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180743 WP_004150355.1 NZ_CP064252:c5407543-5407301 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180743 NZ_CP085696:200668-200955 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References