Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91733..92376 Replicon plasmid pJX2-2
Accession NZ_CP064248
Organism Klebsiella pneumoniae strain JX-CR-hvKP-2

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS498_RS29075 Protein ID WP_001044770.1
Coordinates 91733..92149 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS498_RS29080 Protein ID WP_001261282.1
Coordinates 92146..92376 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS498_RS29055 87836..88108 - 273 Protein_109 transposase -
IS498_RS29060 88137..88834 - 698 Protein_110 IS1 family transposase -
IS498_RS29065 89090..90112 - 1023 WP_000361404.1 helicase UvrD -
IS498_RS29070 90097..91659 - 1563 WP_004206609.1 AAA family ATPase -
IS498_RS29075 91733..92149 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS498_RS29080 92146..92376 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS498_RS29085 92333..92794 + 462 WP_014343465.1 hypothetical protein -
IS498_RS29090 92955..93899 + 945 WP_011977810.1 hypothetical protein -
IS498_RS29095 93936..94328 + 393 WP_011977811.1 hypothetical protein -
IS498_RS29100 94386..94907 + 522 WP_013214008.1 hypothetical protein -
IS498_RS29105 94953..95156 + 204 WP_011977813.1 hypothetical protein -
IS498_RS29110 95186..96190 + 1005 WP_011977814.1 hypothetical protein -
IS498_RS29115 96374..97153 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..168025 168025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180724 WP_001044770.1 NZ_CP064248:c92149-91733 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180724 NZ_CP085638:4768295-4768606 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180724 WP_001261282.1 NZ_CP064248:c92376-92146 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180724 NZ_CP085638:4768607-4768897 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References