Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 142586..143111 Replicon plasmid pJX3-2
Accession NZ_CP064243
Organism Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS502_RS28500 Protein ID WP_001159871.1
Coordinates 142586..142891 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS502_RS28505 Protein ID WP_000813634.1
Coordinates 142893..143111 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS502_RS28460 138338..138565 + 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -
IS502_RS28465 138599..138964 + 366 WP_021519752.1 type IV conjugative transfer system pilin TraA -
IS502_RS28470 138979..139290 + 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
IS502_RS28475 139312..139878 + 567 WP_000399794.1 type IV conjugative transfer system protein TraE -
IS502_RS28480 139889..140593 + 705 WP_072095570.1 type-F conjugative transfer system secretin TraK -
IS502_RS28485 140593..141330 + 738 Protein_183 conjugal transfer protein TraB -
IS502_RS28490 141382..142086 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
IS502_RS28495 142151..142585 - 435 Protein_185 tyrosine-type recombinase/integrase -
IS502_RS28500 142586..142891 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS502_RS28505 142893..143111 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS502_RS28510 143670..144596 + 927 WP_001290414.1 hypothetical protein -
IS502_RS28515 144646..144900 + 255 WP_000678528.1 hypothetical protein -
IS502_RS28520 145398..145742 + 345 WP_000792769.1 hypothetical protein -
IS502_RS28525 145916..146917 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS502_RS28530 147490..147780 - 291 WP_000648897.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / blaSHV-182 - 1..154868 154868
- flank IS/Tn - - 141382..142086 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180696 WP_001159871.1 NZ_CP064243:c142891-142586 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180696 NZ_CP085637:1973707-1973810 [Escherichia coli]
GGCAAGGCAACTAAGCCTGTATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180696 WP_000813634.1 NZ_CP064243:c143111-142893 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180696 NZ_CP085637:c1973848-1973577 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATACAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References