180696

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 142586..143111 Replicon plasmid pJX3-2
Accession NZ_CP064243
Organism Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS502_RS28500 Protein ID WP_001159871.1
Coordinates 142586..142891 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS502_RS28505 Protein ID WP_000813634.1
Coordinates 142893..143111 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS502_RS28460 138338..138565 + 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -
IS502_RS28465 138599..138964 + 366 WP_021519752.1 type IV conjugative transfer system pilin TraA -
IS502_RS28470 138979..139290 + 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
IS502_RS28475 139312..139878 + 567 WP_000399794.1 type IV conjugative transfer system protein TraE -
IS502_RS28480 139889..140593 + 705 WP_072095570.1 type-F conjugative transfer system secretin TraK -
IS502_RS28485 140593..141330 + 738 Protein_183 conjugal transfer protein TraB -
IS502_RS28490 141382..142086 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
IS502_RS28495 142151..142585 - 435 Protein_185 tyrosine-type recombinase/integrase -
IS502_RS28500 142586..142891 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS502_RS28505 142893..143111 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS502_RS28510 143670..144596 + 927 WP_001290414.1 hypothetical protein -
IS502_RS28515 144646..144900 + 255 WP_000678528.1 hypothetical protein -
IS502_RS28520 145398..145742 + 345 WP_000792769.1 hypothetical protein -
IS502_RS28525 145916..146917 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS502_RS28530 147490..147780 - 291 WP_000648897.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / blaSHV-182 - 1..154868 154868
- flank IS/Tn - - 141382..142086 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180696 WP_001159871.1 NZ_CP064243:c142891-142586 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180696 NZ_CP085637:1973707-1973810 [Escherichia coli]
GGCAAGGCAACTAAGCCTGTATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180696 WP_000813634.1 NZ_CP064243:c143111-142893 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180696 NZ_CP085637:c1973848-1973577 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATACAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References