Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 7447..8006 Replicon plasmid pJX3-2
Accession NZ_CP064243
Organism Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag IS502_RS27615 Protein ID WP_000421257.1
Coordinates 7447..7722 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag IS502_RS27620 Protein ID WP_001178089.1
Coordinates 7722..8006 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS502_RS27580 2626..4017 - 1392 WP_000085084.1 SIR2 family protein -
IS502_RS27585 4094..4579 - 486 Protein_3 recombinase family protein -
IS502_RS27590 4643..5347 + 705 WP_001067845.1 IS6-like element IS26 family transposase -
IS502_RS27595 5434..5781 - 348 WP_000142451.1 hypothetical protein -
IS502_RS27600 5910..6245 + 336 WP_001080730.1 colicin 1A immunity protein -
IS502_RS27605 6267..6626 - 360 WP_085452786.1 colicin -
IS502_RS27610 6700..7397 + 698 Protein_8 IS1 family transposase -
IS502_RS27615 7447..7722 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IS502_RS27620 7722..8006 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
IS502_RS27625 8650..9654 - 1005 WP_021546935.1 IS110-like element IS4321 family transposase -
IS502_RS27630 9844..10548 - 705 WP_001067845.1 IS6-like element IS26 family transposase -
IS502_RS27635 10612..10848 - 237 Protein_13 DUF3330 domain-containing protein -
IS502_RS27640 11051..12064 - 1014 WP_000845048.1 class 1 integron integrase IntI1 -
IS502_RS27645 12244..12798 + 555 WP_032488579.1 aminoglycoside N-acetyltransferase AAC(6')-Ib3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / blaSHV-182 - 1..154868 154868
- inside IScluster/Tn cmlA1 / qacE / blaCTX-M-14 - 4643..30110 25467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T180688 WP_000421257.1 NZ_CP064243:c7722-7447 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T180688 NZ_CP085636:c6348657-6348406 [Shewanella sp. MTB7]
ATGAGTGTTTATAGTTTTACTGAGGTAAGAAGTAATTTTAAGTCTGTTTGTGACAGTGTCGTCAATGACTGCGAACCTGC
AGTGATACATCGTAGAGGTGCTGAAAATGTCGTCATGTTCTCTGAGTCAGAGTTCAATAGTTGGAAAGAGACTATTTACT
TACTTTCAAACCCAGTAAATGCAAAACATCTGCTAGATTCTATAAAGCAGGTTGAAGAAGGCCAGGCTCAAGAGCGCGAG
TTACTGGATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT180688 WP_001178089.1 NZ_CP064243:c8006-7722 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT180688 NZ_CP085636:c6348406-6348146 [Shewanella sp. MTB7]
ATGAATATTCTCTTCTCTGATAATGCTTGGAACGATTACCTTTATTGGCAACAACAAGATAAAAAAACATTGAAACGAAT
TAACCTGCTTATTAAAGATTGTCAAAGGACACCTTTTGAAGGCATTGGCAAACCAGAAAGATTAAAAGAAAACCTTTCTG
GTTATTGGTCTAGACGGATCACAGATGAACATCGTTTGGTTTATAAGGCAACTCAGGATAGGTTAACGATTGTTGCCCTA
CGCTATCACTACTCTTCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References