Detailed information of TA system TA180685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 71359..72029
• Replicon: plasmid pJX3-1
• Accession: NZ_CP064242
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS502_RS26765
• Protein ID: WP_004213072.1
• Coordinates: 71586..72029 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS502_RS26760
• Protein ID: WP_004213073.1
• Coordinates: 71359..71589 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS502_RS26735	67583..68482	-	900	WP_004225022.1	nucleotide-binding protein	-
IS502_RS26740	68472..68762	-	291	WP_004213078.1	hypothetical protein	-
IS502_RS26745	69114..69320	+	207	WP_004213077.1	hypothetical protein	-
IS502_RS26750	69310..69603	-	294	WP_004213076.1	hypothetical protein	-
IS502_RS26755	69619..70752	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS502_RS26760	71359..71589	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS502_RS26765	71586..72029	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS502_RS26770	72043..72459	-	417	Protein_78	restriction endonuclease	-
IS502_RS26775	72463..72756	-	294	Protein_79	transposase	-
IS502_RS26780	73173..73808	+	636	Protein_80	response regulator	-
IS502_RS26785	74323..74729	-	407	Protein_81	GAF domain-containing protein	-
IS502_RS26790	74820..75740	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS502_RS26795	75789..76280	-	492	WP_011251327.1	DM13 domain-containing protein	-
IS502_RS26800	76343..76618	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..221242	221242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180685 WP_004213072.1 NZ_CP064242:71586-72029 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180685 NZ_CP085636:4235369-4235767 [Shewanella sp. MTB7]
ATGCTTAAATACATGCTTGATACCAATATTTGTATTTATGTCATCAAGAAAAAACCTTTGGAAGCATTGCAAGCTTTTAA
CCTTCATGCAGGACAGCTATGTATAAGTTCTATCACGTTAGCTGAACTTTTACACGGAGCATCGAAAAGTGTAAAACCAG
AGCATAACTTACGAATTGTAGAAGACTTCGTTTCCCGCCTTGATGTTTTGAATTACGGCGCTAAGGCAGCCTCTCATTAT
GGTGACATTCGAGCGAATTTAGAGCGGAAAGGCACACCTATAGGAGTAAATGATCTCCACATTAGTGGTCATGCTAGAAG
TGAAGCTTTAATCATAGTAACAAACAACGAAAAAGAATTTATTCGTGTAGACGGATTACGAGTTGAAAACTGGGTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180685 WP_004213073.1 NZ_CP064242:71359-71589 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180685 NZ_CP085636:4235135-4235368 [Shewanella sp. MTB7]
ATGAGTGTAGCATCAGTTTTTATTAACAACCGAACCCAAGCGGTACGTTTACCCGTTGACAGTCGTTTTCCTGAAGATGT
TAAGAAGGTCAACGTCAGAGTCGTAGGTAAGGAGCGGATACTTTCACCGATTGACAATACATGGGATAGTTTCTTCCTGC
CACAAACTTCCGTGACAGACGACTTCATGGCAGAACGCGCAAGTCAAGAACAAGCCGAGAGGGAATCGTTCTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620