Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4717078..4717888 Replicon chromosome
Accession NZ_CP064241
Organism Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IS502_RS23245 Protein ID WP_002887280.1
Coordinates 4717078..4717611 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IS502_RS23250 Protein ID WP_002887278.1
Coordinates 4717622..4717888 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS502_RS23240 4715909..4717030 + 1122 WP_002887282.1 cupin domain-containing protein -
IS502_RS23245 4717078..4717611 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
IS502_RS23250 4717622..4717888 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IS502_RS23255 4717991..4719424 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
IS502_RS23260 4719414..4720097 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IS502_RS23265 4720269..4721654 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
IS502_RS23270 4721672..4722016 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T180681 WP_002887280.1 NZ_CP064241:c4717611-4717078 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T180681 NZ_CP085633:c4537431-4536544 [Pectobacterium brasiliense]
ATGCTGAAGCAACAAATTCGCCAAATCGTCCAGTCAAACCCTGACTATGCCGCAGTGACACCCGTCATAGAAAAAGAGAT
CTTGCATCATGACATTATAGATGTGCTGATCAAACAGGGTGTCATGCAACGGTTAACGTTTATTGGCGGCACCTCATTAC
GAATGTGTTACAACAGTAGCAGGCTATCAGAAGATCTTGATTTCAATGGTGGTCATGATTTCAAGCCCTCAGCATTTGAG
GGGCTGGAATCCGAGATCCAAACCTATATTCAGAACAAGTATGAAACCGAAGTTTGGGTAAACAAACCTTCTGGCGAGCG
GCAAGGGGATACCGTTTCTTGGAAAATTAGTATCGTAAAAGAAGCGAATCGTCCTGATTTGCCACGGCAAAAAATGCATA
TTGATGTCTGTGCTATTCCATCATTTGATATTGAAAAGCGCCCGCTAATCAATCATTACGACATTGTTGTACCGACTGAA
GGGATCTTGGTTCCGGTACAATCGTTACAAGAAACACTAGCCGATAAGCTGATCGCGTTAGCGTATCGGGCAAGACGTAT
AAAGCCTCGTGATATCTGGGATATTGTTTGGATTAAGCAGCGCGGCATTGATCTATCCAAAACACTGGTTGAAAAGAAAC
TCACCGCCAGACACAAGCAAACAGATGACTTTCGACAGGCGCTATCTATTCAGTTAGCTAAGTTAATGTTGCAAGATGAA
GTCCGTGACGACTTTAATATGGAAATGAGCCGCTTTATCCCTAAGCAAATTAAAGAGAGAACCCTCGATAATCCCGAATA
TTGGGATTATGTTCAGAGAGAGGTACATGCCATCACGTCTGAGTTATTGAATGACGTAACACCAAAAAATCCATTTGATA
TGGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT180681 WP_002887278.1 NZ_CP064241:c4717888-4717622 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT180681 NZ_CP085633:c4537984-4537460 [Pectobacterium brasiliense]
ATGACTAAATCTGATGAGCTACTAAACGTATTTTCCAAAGCGGTGAACGCTGGCGGTGGCATTCACAGTGCAACAGAGCT
GGCTTTTATGCAGGGTGTGCCATGTGACTCAGCCTTTAGAAAGTTCCTAGCCGATTGCGTAAAGAAGGGATTGCTAAGAC
GAGTGGTGAAAGGCTTCTATGAAAGTGTTATTACACCACCTGAGCCTGAGACTGCCATTTATAAAATCATCAAAAAGTTG
CGCAGTGGCGTATTGAACTACATCAGTTTAGAAAGCCAGCTCAGTCATACCGGTGATATTTCACAAGTTGTGATGGGTAG
AGTCACGGTAGTGACTAAAGGTCGAAGTGGTTGTTTTGATACGCCTTATGGCGTGATCGAGTTTACCCATACTAAAAAGC
CAGTCGAGCAAGTTGCACCTAATTTGTACTATGACCCTGACATTAAAATGTATCGCGCATACAAGGAACAAGCGATTGCA
GATCTAAAGCACTGCCAGCGCAATCTACATATGTTGGAGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References