Detailed information of TA system TA180666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1396..1870
• Replicon: plasmid pJX4-5
• Accession: NZ_CP064240
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: IS499_RS29785
• Protein ID: WP_004197762.1
• Coordinates: 1604..1870 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: IS499_RS29780
• Protein ID: WP_000879771.1
• Coordinates: 1396..1620 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS499_RS29770	1..510	+	510	WP_032433972.1	MbeB family mobilization protein	-
IS499_RS29775	517..729	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
IS499_RS29780	1396..1620	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
IS499_RS29785	1604..1870	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS499_RS29790	1940..2290	-	351	WP_004197756.1	hypothetical protein	-
IS499_RS29795	2356..2901	-	546	WP_023317533.1	hypothetical protein	-
IS499_RS29800	2927..3306	-	380	Protein_6	hypothetical protein	-
IS499_RS29805	4409..4585	+	177	WP_004197757.1	hypothetical protein	-
IS499_RS29810	4575..4919	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
IS499_RS29815	4858..5556	+	699	Protein_9	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T180666 WP_004197762.1 NZ_CP064240:1604-1870 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T180666 NZ_CP085633:150547-150822 [Pectobacterium brasiliense]
ATGCAGGGAAAGATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGAGCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAATATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAGGTAGCGGATCACGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGGTTCCATCGTCCCCATCCGTCACCGGATACTGATAAAGGTGCACTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT180666 WP_000879771.1 NZ_CP064240:1396-1620 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT180666 NZ_CP085633:150819-151181 [Pectobacterium brasiliense]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCGGCCATTATCAGTTACGTACCGGAAATGGG
TATGTTTCGCGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGCTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGCTACCCTGAATCATTCGGGGAACGACTGACCATTGCGGCCGCTGAACGCCAAATTTCAGTGAATAC
GTTTATTGTTGAAACGCTTAGCGAACACCTGAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5