Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39680..40205 Replicon plasmid pJX4-2
Accession NZ_CP064237
Organism Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS499_RS28390 Protein ID WP_001159871.1
Coordinates 39900..40205 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS499_RS28385 Protein ID WP_000813634.1
Coordinates 39680..39898 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS499_RS28360 35011..35301 + 291 WP_000648897.1 hypothetical protein -
IS499_RS28365 35874..36875 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS499_RS28370 37049..37393 - 345 WP_000792769.1 hypothetical protein -
IS499_RS28375 37891..38145 - 255 WP_000678528.1 hypothetical protein -
IS499_RS28380 38195..39121 - 927 WP_001290414.1 hypothetical protein -
IS499_RS28385 39680..39898 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS499_RS28390 39900..40205 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS499_RS28395 40206..40640 + 435 Protein_48 tyrosine-type recombinase/integrase -
IS499_RS28400 40705..41409 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
IS499_RS28405 41461..42198 - 738 Protein_50 conjugal transfer protein TraB -
IS499_RS28410 42198..42902 - 705 WP_072095570.1 type-F conjugative transfer system secretin TraK -
IS499_RS28415 42913..43479 - 567 WP_000399794.1 type IV conjugative transfer system protein TraE -
IS499_RS28420 43501..43812 - 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
IS499_RS28425 43827..44192 - 366 WP_021519752.1 type IV conjugative transfer system pilin TraA -
IS499_RS28430 44226..44453 - 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / blaCTX-M-14 / blaSHV-12 / blaKPC-2 / blaTEM-1B - 1..165027 165027
- flank IS/Tn - - 40705..41409 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180656 WP_001159871.1 NZ_CP064237:39900-40205 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180656 NZ_CP085625:c43052-42903 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180656 WP_000813634.1 NZ_CP064237:39680-39898 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180656 NZ_CP085625:43096-43157 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References