Detailed information of TA system TA180654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 193215..193885
• Replicon: plasmid pJX4-1
• Accession: NZ_CP064236
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS499_RS28015
• Protein ID: WP_004213072.1
• Coordinates: 193215..193658 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS499_RS28020
• Protein ID: WP_004213073.1
• Coordinates: 193655..193885 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS499_RS27980	188624..188899	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IS499_RS27985	188962..189453	+	492	WP_011251327.1	DM13 domain-containing protein	-
IS499_RS27990	189502..190422	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS499_RS27995	190513..190919	+	407	Protein_203	GAF domain-containing protein	-
IS499_RS28000	191434..192071	-	638	Protein_204	response regulator	-
IS499_RS28005	192488..192781	+	294	Protein_205	transposase	-
IS499_RS28010	192785..193201	+	417	Protein_206	restriction endonuclease	-
IS499_RS28015	193215..193658	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS499_RS28020	193655..193885	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS499_RS28025	194493..195626	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS499_RS28030	195642..195935	+	294	WP_004213076.1	hypothetical protein	-
IS499_RS28035	195925..196131	-	207	WP_004213077.1	hypothetical protein	-
IS499_RS28040	196483..196773	+	291	WP_004213078.1	hypothetical protein	-
IS499_RS28045	196763..197662	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..219405	219405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180654 WP_004213072.1 NZ_CP064236:c193658-193215 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180654 NZ_CP085622:c4576809-4576498 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180654 WP_004213073.1 NZ_CP064236:c193885-193655 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180654 NZ_CP085622:c4576497-4576207 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620