Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 154325..155235 Replicon plasmid pJX4-1
Accession NZ_CP064236
Organism Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag IS499_RS27780 Protein ID WP_004026354.1
Coordinates 154325..154795 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag IS499_RS27785 Protein ID WP_004026357.1
Coordinates 154792..155235 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS499_RS27760 149737..150135 - 399 WP_004212796.1 H-NS histone family protein -
IS499_RS27765 150472..150966 - 495 WP_004212794.1 thermonuclease family protein -
IS499_RS27770 151144..152093 + 950 Protein_158 DUF1173 family protein -
IS499_RS27775 153479..154176 - 698 Protein_159 IS1 family transposase -
IS499_RS27780 154325..154795 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
IS499_RS27785 154792..155235 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
IS499_RS27790 155336..155839 - 504 Protein_162 DUF4113 domain-containing protein -
IS499_RS27795 156045..157025 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
IS499_RS27800 157091..158398 + 1308 Protein_164 FepA family TonB-dependent siderophore receptor -
IS499_RS27805 158460..158681 + 222 WP_004213615.1 hypothetical protein -
IS499_RS27810 158824..159726 - 903 WP_004213613.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..219405 219405
- inside IScluster/Tn - - 148286..157025 8739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T180653 WP_004026354.1 NZ_CP064236:c154795-154325 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T180653 NZ_CP085622:c4298467-4298294 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT180653 WP_004026357.1 NZ_CP064236:c155235-154792 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT180653 NZ_CP085622:c4298791-4298495 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References