Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5398944..5399569 Replicon chromosome
Accession NZ_CP064235
Organism Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS499_RS26615 Protein ID WP_002882817.1
Coordinates 5398944..5399327 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS499_RS26620 Protein ID WP_004150355.1
Coordinates 5399327..5399569 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS499_RS26600 5396310..5397212 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS499_RS26605 5397209..5397844 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS499_RS26610 5397841..5398770 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS499_RS26615 5398944..5399327 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS499_RS26620 5399327..5399569 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS499_RS26625 5399774..5400691 + 918 WP_002882812.1 alpha/beta hydrolase -
IS499_RS26630 5400705..5401646 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS499_RS26635 5401691..5402128 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS499_RS26640 5402125..5402985 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS499_RS26645 5402979..5403578 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180651 WP_002882817.1 NZ_CP064235:c5399327-5398944 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180651 NZ_CP085622:3715110-3715388 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180651 WP_004150355.1 NZ_CP064235:c5399569-5399327 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180651 NZ_CP085622:3714847-3715107 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References