Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46080..46723 Replicon plasmid pJX5-2
Accession NZ_CP064234
Organism Klebsiella pneumoniae strain JX-CR-hvKP-5

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS503_RS27415 Protein ID WP_001044770.1
Coordinates 46307..46723 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS503_RS27410 Protein ID WP_001261282.1
Coordinates 46080..46310 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS503_RS27370 41408..41587 + 180 WP_006788217.1 hypothetical protein -
IS503_RS27375 42043..42837 - 795 WP_004197635.1 site-specific integrase -
IS503_RS27380 43035..44051 - 1017 WP_017899884.1 hypothetical protein -
IS503_RS27385 44062..44376 - 315 WP_053389906.1 hypothetical protein -
IS503_RS27390 44403..44798 - 396 WP_017899885.1 hypothetical protein -
IS503_RS27395 44967..45272 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
IS503_RS27400 45274..45492 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
IS503_RS27405 45662..46123 - 462 WP_160880186.1 hypothetical protein -
IS503_RS27410 46080..46310 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS503_RS27415 46307..46723 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS503_RS27420 46797..48359 + 1563 WP_004206609.1 AAA family ATPase -
IS503_RS27425 48344..49366 + 1023 WP_000361404.1 helicase UvrD -
IS503_RS27430 49622..50319 + 698 Protein_70 IS1 family transposase -
IS503_RS27435 50348..50620 + 273 Protein_71 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..105085 105085


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180633 WP_001044770.1 NZ_CP064234:46307-46723 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180633 NZ_CP085622:270364-270594 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180633 WP_001261282.1 NZ_CP064234:46080-46310 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180633 NZ_CP085622:270591-270950 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References