Detailed information of TA system TA180629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 86111..86781
• Replicon: plasmid pJX5-1
• Accession: NZ_CP064233
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS503_RS26315
• Protein ID: WP_004213072.1
• Coordinates: 86338..86781 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS503_RS26310
• Protein ID: WP_004213073.1
• Coordinates: 86111..86341 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS503_RS26285	82334..83233	-	900	WP_004225022.1	nucleotide-binding protein	-
IS503_RS26290	83223..83513	-	291	WP_004213078.1	hypothetical protein	-
IS503_RS26295	83865..84071	+	207	WP_004213077.1	hypothetical protein	-
IS503_RS26300	84061..84354	-	294	WP_004213076.1	hypothetical protein	-
IS503_RS26305	84370..85503	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS503_RS26310	86111..86341	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS503_RS26315	86338..86781	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS503_RS26320	86795..87211	-	417	Protein_93	restriction endonuclease	-
IS503_RS26325	87215..87508	-	294	Protein_94	transposase	-
IS503_RS26330	87925..88560	+	636	Protein_95	response regulator	-
IS503_RS26335	89075..89481	-	407	Protein_96	GAF domain-containing protein	-
IS503_RS26340	89572..90492	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS503_RS26345	90541..91032	-	492	WP_011251327.1	DM13 domain-containing protein	-
IS503_RS26350	91095..91370	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..228973	228973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180629 WP_004213072.1 NZ_CP064233:86338-86781 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180629 NZ_CP085619:c4141929-4141552 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180629 WP_004213073.1 NZ_CP064233:86111-86341 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180629 NZ_CP085619:c4142386-4142018 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620