Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5327543..5328168 Replicon chromosome
Accession NZ_CP064232
Organism Klebsiella pneumoniae strain JX-CR-hvKP-5

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag IS503_RS25490 Protein ID WP_012737091.1
Coordinates 5327543..5327926 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS503_RS25495 Protein ID WP_004150355.1
Coordinates 5327926..5328168 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS503_RS25475 5324909..5325811 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS503_RS25480 5325808..5326443 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS503_RS25485 5326440..5327369 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS503_RS25490 5327543..5327926 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
IS503_RS25495 5327926..5328168 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS503_RS25500 5328373..5329290 + 918 WP_012737090.1 alpha/beta hydrolase -
IS503_RS25505 5329304..5330245 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS503_RS25510 5330290..5330727 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS503_RS25515 5330724..5331584 - 861 WP_004146232.1 virulence factor BrkB family protein -
IS503_RS25520 5331578..5332177 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T180628 WP_012737091.1 NZ_CP064232:c5327926-5327543 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180628 NZ_CP085619:c4071272-4070985 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180628 WP_004150355.1 NZ_CP064232:c5328168-5327926 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180628 NZ_CP085619:c4070998-4070696 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References