Detailed information of TA system TA180624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2727310..2728898
• Replicon: chromosome
• Accession: NZ_CP064232
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-5

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A331XLA5
• Locus tag: IS503_RS13155
• Protein ID: WP_014907613.1
• Coordinates: 2727310..2728632 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: IS503_RS13160
• Protein ID: WP_002904790.1
• Coordinates: 2728632..2728898 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS503_RS13140	2722366..2724867	+	2502	WP_014907611.1	fimbrial biogenesis outer membrane usher protein	-
IS503_RS13145	2724855..2725859	+	1005	WP_014907612.1	type 1 fimbrial protein	-
IS503_RS13150	2726001..2727293	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
IS503_RS13155	2727310..2728632	-	1323	WP_014907613.1	type II toxin-antitoxin system HipA family toxin	Toxin
IS503_RS13160	2728632..2728898	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IS503_RS13165	2729136..2729462	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
IS503_RS13170	2729459..2729959	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
IS503_RS13175	2729937..2731316	-	1380	WP_004184021.1	purine permease	-
IS503_RS13180	2731662..2732816	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
IS503_RS13185	2732797..2733720	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T180624 WP_014907613.1 NZ_CP064232:c2728632-2727310 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T180624 NZ_CP085619:c3497599-3497297 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT180624 WP_002904790.1 NZ_CP064232:c2728898-2728632 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT180624 NZ_CP085619:c3497261-3496920 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A331XLA5

Antitoxin

• AlphaFold DB: A0A0H3GXD8